Composite

Part:BBa_K3257100:Design

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-23)


Improved LacI Expression System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1258
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1258
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

lacIq promoter and rrnB terminator are both the endogenous biobricks that can be found in Escherichia coli or other bacteria so that this system works well in E.coli BL21(DE3).

Source

Combined from BBa_K3257003, BBa_K3257045, BBa_K3257200 and BBa_K3257020.

References

AJ Meyer et al. Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Nat Chem Biol. 2019 Feb;15(2):196-204. doi: 10.1038/s41589-018-0168-3. Epub 2018 Nov 26.